Novel Technique for the Preparation of Corneal Grafts for Descemet Membrane Endothelial Keratoplasty – Corrected Proof

Purpose: To report a simple novel technique to facilitate preparation of Descemet membrane grafts for Descemet membrane endothelial keratoplasty (DMEK).Design: Laboratory investigation and retrospective, single-center, consecutive case series.Methods: Preparation of the endothelial graft is performed on an artificial anterior chamber, endothelial side up. After an incomplete circular superficial trephination, we describe a simple technique using a 27 gauge cannula to detach the Descemet membrane (DM). Endothelial cell density (ECD) was measured before dissection on 12 human corneas for research and 3 days after storage in organ culture. Histologic and electron microscopy analysis were performed. A DMEK was performed in 50 patients (Read more...)

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