Atomic force microscopy shows reduced elasticity of prolonged cold-stored DMEK graft

Longer graft storage time in cold media significantly reduced the elasticity of a Descemet’s membrane endothelial keratoplasty graft, with potential effects on unfolding within the anterior chamber as well as postoperative detachment rate.
“Unfolding difficulties with tight grafts are one of the most common challenges of DMEK,” Natalya Shilova, MD, said at the virtual European Society of Cataract and Refractive Surgeons meeting.
In the study Shilova performed with her group, atomic force microscopy (AFM) was used to measure the effect of graft storage time on the elastic

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