Differential Sensitivity of Choroidal Endothelial, Retinal Ganglion, and Retinal Pigment Epithelial Cells In Vitro to Proton Radiation – Corrected Proof
Purpose: To evaluate the differential sensitivity of choroidal endothelial, retinal pigment epithelial, and retinal ganglion cells to escalating doses of proton beam radiation and to establish a safe dose range for the management of choroidal neovascularization associated with age-related macular degeneration (AMD).Design: Laboratory investigation.Methods: Proliferating simian choroidal endothelial cells (RF/6A), differentiated rat retinal ganglion cells (RGC-5), and serum-starved human retinal pigment epithelial cells (ARPE-19) were exposed to 2, 4, 8, and 12 cobalt gray equivalent of proton beam radiation and cell viability was quantified on day 9. Reactive oxygen species levels were analyzed.Results: Significant decline of choroidal endothelial cell viability (Read more...)