Reproducibility of Graft Preparations in Descemet’s Membrane Endothelial Keratoplasty – Corrected Proof

Purpose: To assess the reproducibility of manual graft preparation and evaluate the incidence rate and nature of structural anomalies of Descemet's membrane (DM) preventing successful graft preparation in DM endothelial keratoplasty (DMEK).Design: Prospective, single-center, nonrandomized, consecutive case series.Participants: We analyzed 350 corneoscleral buttons from donors aged 18–95 years stored in Optisol-GS or Dulbecco's modified Eagle's medium and used for DMEK surgery in 343 consecutive patients with Fuchs' endothelial dystrophy or pseudophakic bullous keratopathy.Methods: Residual endothelial cell–DM complexes obtained after successful DM stripping for DMEK and whole donor corneas obtained after unsuccessful DM stripping were examined by transmission electron microscopy and (Read more...)

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