KRT12 Mutations and In Vivo Confocal Microscopy in Two Japanese Families With Meesmann Corneal Dystrophy – Corrected Proof
Purpose: To identify genetic mutations and study the corneal epithelium in Japanese patients with Meesmann corneal dystrophy.Design: Laboratory investigation and prospective observational case series.Methods: Slit-lamp biomicroscopy with fluorescein vital staining and in vivo confocal microscopy were performed. Mutation screening of the KRT3 and KRT12 genes was performed via polymerase chain reaction and direct sequencing for 5 patients in 2 families.Results: Slit-lamp biomicroscopy revealed multiple corneal intraepithelial microcysts in all patients. A clear zone was seen in the younger generation, whereas mild subepithelial opacity was seen in the older generation. In the in vivo confocal microscopy, numerous corneal intraepithelial microcysts and hyperreflective materials, (Read more...)