Purpose: To investigate the in vitro effect of pH, osmolarity, solvent, and light interaction on currently used and novel dyes to minimize dye-related retinal toxicity.Design: Laboratory investigation.Methods: Retinal pigment epithelium (RPE) human cells (ARPE-19) were exposed for 10 minutes to different pH solutions (4, 5, 6, 7, 7.5, 8, and 9) and glucose solutions (2.5%, 5.0%, 10%, 20%, 40%, and 50%) with osmolarity from 142 to 2530 mOsm, with and without 0.5 mg/mL trypan blue. R28 cells were also incubated with glucose (150, 310, and 1000 mOsm) and mannitol used as an osmotic control agent in both experiments. Dye-light interaction was assessed by (Read more...)
Author: Am J Ophthalmol
Ocular Biometry in the Subtypes of Angle Closure: An Anterior Segment Optical Coherence Tomography Study
Purpose: To evaluate ocular biometric parameters in different subtypes of angle-closure disease in the Iranian population and compare them with normal eyes.Design: Prospective, cross-sectional.Methods: In this clinic-based study, 189 eyes of 154 patients consisting of 40 acute angle-closure glaucoma (AACG) eyes, 40 fellow eyes of AACG, 42 chronic angle-closure glaucoma (CACG) eyes, 40 primary angle-closure suspect (PACS) eyes, and 27 normal eyes underwent complete examination including gonioscopy, A-scan biometry, and anterior segment optical coherence tomography. Only 1 eye of CACG, PACS, and control subjects were selected. Main outcome measures included angle opening distance and trabeculo-iris space area at 500 μm from (Read more...)
Prevalence and Risk Factors for Age-Related Macular Degeneration in Indians: A Comparative Study in Singapore and India
Purpose: To compare the prevalence and risk factors for age-related macular degeneration (AMD) in 2 Indian populations, 1 living in urban Singapore and 1 in rural central India.Design: Population-based, cross-sectional studies of Indians aged 40+ years…
Risk of Endophthalmitis and Other Long-Term Complications of Trabeculectomy in the Collaborative Initial Glaucoma Treatment Study (CIGTS)
Purpose: To report the risk of endophthalmitis and other long-term complications in patients randomized to trabeculectomy in the Collaborative Initial Glaucoma Treatment Study.Design: A longitudinal cohort study using data collected from a multicenter,…
Intravitreal Anti–Vascular Endothelial Growth Factor Therapy Versus Photodynamic Therapy for Idiopathic Choroidal Neovascularization
Purpose: To compare visual outcomes after intravitreal anti–vascular endothelial growth factor (VEGF) injection or photodynamic therapy (PDT) for idiopathic choroidal neovascularization (CNV).Design: Retrospective study.Methods: Among 29 eyes (28 pat…
Ultrasound Biomicroscopy of the Ciliary Body in Ocular/Oculodermal Melanocytosis
Purpose: To describe the ultrasound biomicroscopy (UBM) findings of the ciliary body in patients with ocular/oculodermal melanocytosis.Design: Retrospective observational case series study.Methods: A retrospective chart and imaging database review was conducted for patients with unilateral ocular/oculodermal melanocytosis who underwent UBM examination at the Ocular Oncology Clinic of Princess Margaret Hospital. Radial images of the ciliary body at the 3-, 6-, 9-, and 12-o’clock positions were obtained in both eyes. UBM characteristics included ciliary body thickness and reflectivity. The eye with ocular/oculodermal melanocytosis was compared with the contralateral unaffected eye as a control. Statistical significance was analyzed with Student t test.Results: (Read more...)
Inadvertent Trypan Blue Posterior Capsule Staining During Cataract Surgery
Purpose: To report 5 cases of inadvertent posterior capsule staining with trypan blue during phacoemulsification.Design: Retrospective, observational case series.Methods: Five cases of posterior capsule staining with trypan blue were identified from ca…
Diffusion of Technologies for the Care of Older Adults With Exudative Age-Related Macular Degeneration
Purpose: To determine patterns of diffusion of diagnostic tests and therapeutic interventions in the United States through 2010 for patients with newly diagnosed exudative macular degeneration (AMD).Design: Retrospective longitudinal cohort analysis.Me…
PROSE Therapy Used to Minimize Corneal Trauma in Patients With Corneal Epithelial Defects
Purpose: To review the effectiveness of continuous or extended daily wear of Prosthetic Replacement of the Ocular Surface Ecosystem (PROSE) for treating recurrent or persistent corneal epithelial defects from a variety of causes that were refractory to…
Treatment of Diabetic Macular Edema With a Designed Ankyrin Repeat Protein That Binds Vascular Endothelial Growth Factor: A Phase I/II Study
Purpose: To evaluate the safety and bioactivity of MP0112, a designed ankyrin repeat protein (DARPin) that specifically binds vascular endothelial growth factor (VEGF) in patients with diabetic macular edema (DME). DARPins are a novel class of proteins selected for specific, high-affinity binding to a target protein.Design: Phase I/II, open-label, multicenter dose-escalation trial.Methods: After a single intravitreal injection of MP0112, the main outcomes were safety assessments, aqueous MP0112 levels, change in best-corrected visual acuity (BCVA), and foveal thickness measured by optical coherence tomography. Six cohorts were planned, but only 3 were enrolled (0.04, 0.15, 0.4 mg), because a maximally tolerated dose of (Read more...)